Molecular Analysis of the Single Cell: Importance in the Study of Psychiatric Disorders

James Eberwine, Peter Crino, Steve Arnold, John Trojanowski, Scott Hemby
Departments of Pharmacology
University of Pennsylvania Medical Center
36th and Hamilton Walk
Philadelphia, PA. 19104-6084
215-898-0420
215-573-2236 (Fax)
eberwine@mscf.med.upenn.edu


REFERENCES

1. Abood M, Eberwine J, Erdelyi E, Evans C. Regulation of both preproenkephalin mRNA and its derived opioids by haloperidol–A Method for measurement of peptides and mRNA in the same tissue extract. Mol Brain Res 1990;8:243-248.

2. Akbarian S, Kim J, Potkin S, et al. Gene expression of glutamic acid decarboxylase is reduced without loss of neurons in prefrontal cortex of schizophrenics. Arch Gen Psychiatry 1995;52:258-266.

3. Arnold S, Trojanowski J. Recent advances in defining the neuropathology of schizophrenia. Acta Neuropathol 1996;92:217-231.

4. Buchhalter J, Dichter M. Electrophysiological comparison of pyramidal and stellate nonpyramidal neurons in dissociated cell culture of rat hippocampus. Brain Res Bull 1991;26 333-338.

5. Crino P, Dichter M, Trojanowski J, Eberwine J. Single cell molecular pathology: An analysis of tuberous sclerosis. Proc Natl Acad Sci USA 1996;93:14152-14157.

6. Crino P, Eberwine J. Molecular characterization of the dendritic growth cone: Regulated mRNA transport and local protein synthesis. Neuron 1996;17:1173-1187.

7. Eberwine J, Crino P Dichter, M. Single Cell mRNA amplification: Basic science and clinical implications. Neuroscientist 1995;1:200-211.

8. Eberwine J, Yeh H, Miyashiro K, et al. Analysis of gene expression in single live neurons. Proc Natl Acad Sci USA 1992;89:3010-3014.

8a. Jirikowski G, Sanna P, Bloom F, mRNA coding for oxytocin presented in axons of the hypothalamo-neurohypophysical tract. Proc Natl Acad Sci USA 1990;87:7400-7404.

9. Joyce J, Shane A, Lexow N, Winokur A, Casanova M, Kleinman J. Serotonin uptake sites and serotonin receptors are altered in the limbic system of schizophrenics. Neuropsychopharmacology 1993;8:315-336.

10. Kleinman J, Casanova M, Jaskiw G. The neuropathology of schizophrenia. Schizophr Bull 1988;14:209-216.

11. Liang P, Pardee A. Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction. Science 1992;257:967-971.

12. Lieberman J, Kane J, Johns C. Clozapine: Guidelines for clinical management. J Clin Psychiatry 1989;50:329-338.

13. McGuffin P, Murray R, Reveley A. Genetic influences on the psychoses. Br Med Bull 1987;43:542-556.

14. Miyashiro K, Dichter M, Eberwine J. On the nature and distribution of mRNAs in hippocampal neurites: Implications for neuronal functioning. Proc Natl Acad Sci USA 1994;91:10800-10804.

15. Nasrallah H, Weinberger D. (eds) The neurology of schizophrenia. Amsterdam: Elsevier, 1986.

16. Surmeier D, Wilson D, Eberwine J. Patch-clamp techniques for studying potassium currents in mammalian brain neurons. In: Ion channels of excitable cells. T Narahashi (ed). Methods in Neuro-- Vol.19. Orlando, FL: Academic Press, 1994;39-67.

17. Surmeier J, Eberwine J, Wilson C, Cao Y, Stefani A, Kitai S. Dopamine receptor subtypes colocalize in rat striatonigral neurons. Proc Natl Acad Sci USA 1992;89:10178-10182.

18. Tecott L, Rubenstein J, Paxinos G, Evans C, Eberwine J, Valentino K. Developmental expression of proenkephalin mRNA and peptides in rat striatum. Brain Res Dev Brain Res 1989;49:75-86.

19. Trojanowski J, Arnold S. In pursuit of the molecular neuropathology of schizophrenia. Arch Gen Psychiatry 1995;52:274-276.

20. Van Gelder R, von Zastrow M, Yool A, Dement W, Barchas J, Eberwine J. Amplified RNA (aRNA) synthesized from limited quantities of heterogeneous cDNA. Proc Natl Acad Sci USA 1990;87:1663-1667.

Back to Chapter

published 2000