Figure 2

Representative slot blot containing candidate cDNAs probed with aRNA from hippocampal dendritic growth cone at 72 hours in culture. Candidate cDNAs are linearized, denatured, and blotted onto nylon membrane. These cDNAs will hybridize to any corresponding radiolabeled aRNA in the aRNA population amplified from a growth cone. pBluescript plasmid cDNA is used to determine non-specific background hybridization. Slot A1, CaM Kinase II; A2, activity regulated cytoskeletal associated protein; A3, brain derived neurotrophic factor; A4, MAP2; A5, low molecular weight neurofilament; A6, GAD65; B1, trkA; B2, medium weight neurofilament; B3, nestin; B4, trkC; B5, trk B; B6, a subunit GABA A receptor; C1, NT3; C2, NGFR; C3, nestin; C4, L-type calcium channel; C5, netrin; C6, N-type calcium channel; D5, pBluescript; D6, internexin.

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published 2000