Figure 9. Expression of the mRNA encoding LHRH receptor (LHRH-R) in the adenohypophysis of intact cycling rats and lipopolysaccharide (LPS)-injected rats during the afternoon of proestrus. Top panels display examples of hybridization signal for LHRH-R mRNA in whole 30-mm pituitary sections on X-ray film (Biomax), whereas middle (dark-field) and bottom (bright-field) panels exhibit LHRH-R mRNA positive cells revealed with nuclear NTB-2 emulsion. Note the profound decrease in the expression of LHRH-R mRNA within the adenohypophysis of immune-challenged rats (right panels). (Taken from Nappi and Rivest, ref. 183.)