1. Bentivoglio M, Molinari M. Fluorescent retrograde triple labeling of brainstem reticular neurons. Neurosci Lett 1984;46:121–126.

2. Björklund A, Hökfelt T, Wouterlood FG van den Pol AN, eds. Handbook of chemical neuroanatomy, vol 8. Analysis of neuronal microcircuits and synaptic interaction. Amsterdam: Elsevier, 1990.

3. Bolam JP, Ingham CA. Combined morphological and histochemical techniques for the study of neuronal microcircuits. In: Björklund A, Hökfelt T, Wouterlood FG, van den Pol AN, eds. Handbook of chemical neuroanatomy, vol 8. Analysis of neuronal microcircuits and synaptic interactions. Amsterdam: Elsevier, 1990;125–198.

4. Buhl EH, Schwerdtfeger WK, Germroth P. Intracellular injection of neurons in fixed brain tissue combined with other neuroanatomical techniques at the light and electron microscopic level. In: Björklund A, Hökfelt T, Wouterlood FG, van den Pol AN, eds. Handbook of chemical neuroanatomy, vol 8. Analysis of neuronal microcircuits and synaptic interactions, Amsterdam: Elsevier, 1990;273–304.

5. Chang HT, Kuo H, Whittaker JA, Cooper NG. Light and electron microscopic analysis of projection neurons retrogradely labeled with Fluoro-gold: notes on the application of antibodies to Fluoro-gold. J Neurosci Methods 1990;1:31–37.

6. Chronwall BM, Lewis ME, Schwaber JS, O'Donohue TL. In situ hybridization combined with retrograde fluorescent tract tracing. In: Heimer L, Zaborszky L, eds. Neuroanatomical tract-tracing methods 2. Recent progress. New York: Plenum, 1989;265–295.

7. Cliffer KD, Giesler GJ Jr. Postsynaptic dorsal column pathway of the rat. III. Distribution of ascending afferent fibers. J Neurosci 1989;9:3146–3168.

8. Cullinan WE, Herman JP, Watson SJ. Ventral subicular interaction with the hypothalamic paraventricular nucleus: evidence for a relay in the bed nucleus of the stria terminalis. J Comp Neurol 1993;332:1–20.

9. Freund TF, Somogyi P. Synaptic relationships of Golgi-impregnated neurons as identified by electrophysiological or immunocytochemical techniques. In: Heimer L, Zaborszky L, eds. Neuroanatomical tract-tracing methods 2. Recent progress. New York: Plenum, 1989;201–238.

10. Gall JG, Pardue ML. Formation and detection of RNA–DNA hybrid molecules in cytological preparations. Proc Natl Acad Sci 1969;63:378–383.

11. Gee CE, Roberts JL. In situ hybridization histochemistry: a technique for the study of gene expression in single cells. DNA 1983;2:157–163.

12. Gerfen CR, Sawchenko PE. An anterograde neuroanatomical tracing method that shows the detailed morphology of neurons, their axons and terminals: immunohistochemical localization of an axonally transported plant lectin, Phaseolus vulgaris leucoagglutinin (PHA-L). Brain Res 1984;290:219–238.

13. Gerfen CF, Sawchenko PE, Carlsen J. The PHA-L anterograde axonal tracing method. In: Heimer L, Zaborszky L, eds. Neuroanatomical tract-tracing methods 2. Recent progress. New York: Plenum, 1989;19–47.

14. Groenewegen HJ, Wouterlood FG. Light and electron microscopic tracing of neuronal connections with Phaseolus vulgaris-leucoagglutinin (PHA-L), and combinations with other neuroanatomical techniques. In: Björklund A, Hökfelt T, Wouterlood FG, van den Pol AN, eds. Handbook of chemical neuroanatomy, vol 8. Analysis of neuronal microcircuits and synaptic interactions. Amsterdam: Elsevier, 1990;47–124.

15. Heimer L, Robards MJ, eds. Neuroanatomical tract-tracing methods. New York: Plenum, 1981.

16. Heimer L, Záborszky L, eds. Neuroanatomical tract-tracing methods 2. Recent progress. New York: Plenum, 1989.

17. Herkenham M, Pert CB. Light microscopic localization of brain opiate receptors: a general autoradiographic method which preserves tissue quality. J Neurosci 1982;2:1129–1149.

18. Hsu S, Raine L, Fanger H. Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. J Histochem Cytochem 1981;29:577–580.

19. John HA, Birnstiel ML, Jones KW. RNA-DNA hybrids at the cytological level. Nature 1969;223:582–587.

20. Kitai ST, Penney GR, Chang HT. Intracellular labeling and immunocytochemistry. In: Heimer L, Zaborszky L eds. Neuroanatomical tract-tracing methods 2. Recent progress. New York: Plenum, 1989;173–199.

21. Kuhar MJ, Yamamura HI. Light autoradiographic localization of cholinergic muscarinic receptors in rat brain by specific binding of a potent antagonist. Nature 1975;253:560–561.

22. McQuaid S, Allan GM. Detection protocols for biotinylated probes: optimization using multistep techniques. J Histochem Cytochem 1992;40:569–574.

23. Meo T, Gramsch C, Inan R, Hollt V, Weber E, Herz A, Riethmuller G. Monoclonal antibody to the message sequence Tyr-Gly-Gly-Phe of opioid peptides exhibits the specificity requirements of mammalian opioid receptors. Proc Natl Acad Sci 1983;80:4084–4088.

24. Mesulam M-M, ed. Tracing neural connections with horseradish peroxidase. New York: John Wiley & Sons, 1982.

25. Normand E, Bloch B. Simultaneous detection of two mRNAs in the central nervous system: a simple two-step in situ hybridization procedure using a combination of radioactive and non-radioactive probes. J Histochem Cytochem 1991;39:1575–1578.

26. Marks DL, Wiemann JN, Burton KA, Lent KL, Clifton DK, Steiner RA. Simultaneous visualization of two cellular mRNA species in individual neurons by use of a new double in situ hybridization method. Mol Cell Neurosci 1992;3:395–405.

27. Rogers AW, ed. Techniques of autoradiography, 2nd ed. New York: Elsevier, 1979.

28. Roth LJ, Diab IM, Watanabe M, Dinerstein RJ. A correlative radioautographic, fluorescent, and histochemical technique for cytopharmacology. Mol Pharmacol 1974;10:986–988.

29. Sawchenko PE, Swanson LW. A method for tracing biochemically defined and immunohistochemical techniques. Brain Res 1982; 210:31–52.

30. Schäfer MK-H, Herman JP, Watson SJ. In situ hybridization. In: London ED, ed. Imaging drug action in the brain. Boca Raton, FL: CRC Press, 1993;337–378.

31. Schmued LC, Fallon JH. Fluoro-gold: a new fluorescent retrograde axonal tracer with numerous unique properties. Brain Res 1986;377:147–154.

32. Simmons DM, Arriza JL, Swanson LW. A complete protocol for in situ hybridization of messenger RNAs in brain and other tissues with radio-labeled single-stranded RNA probes. J Histotechnol 1989;12:169–181.

33. Skirboll LR, Thor K, Helke C, Hökfelt T, Robertson B, Long R. Use of retrograde fluorescent tracers in combination with immunohistochemical methods. In: Heimer L, Zaborszky L, eds. Neuroanatomical tract-tracing methods 2. Recent progress. New York: Plenum, 1989.

34. Sternberger LA. Immunocytochemistry, 3rd ed. New York: John Wiley & Sons, 1986.

35. Sternberger LA, Hardy PH, Cuculis JJ, Meyer HG. The unlabeled-enzyme method of immunocytochemistry. Preparation and properties of soluble antigen–antibody complex (horseradish peroxidase-anti-horseradish peroxidase) and its use in identification of spirochetes. J Histochem Cytochem 1970;18:315–333.

36. Swanson LW, Sawchenko PE, Lind RW. Regulation of multiple peptides in CRF parvocellular neurosecretory neurons: implications for the stress response. In: Hökfelt T, Fuxe K, Pernow B, eds. Progress in brain research, vol 68, Amsterdam: Elsevier Science Publishers, 1986;169–190.

37. Uhl GR, ed. In situ hybridization in brain. New York: Plenum, 1986.

38. Valentino KL, Eberwine JH, Barchas JD, eds. In situ hybridization. Applications to neurobiology. New York: Oxford University Press, 1987.

39. Watts AG, Swanson LW. The combination of in situ hybridization with immunocytochemistry and retrograde tract-tracing. In: Conn PM, ed. Methods in neurosciences, vol 1. Genetic probes. New York: Academic Press, 1989;127–136.

40. Webster HF, Lamperth L, Favilla JT, Lemke G, Tesin D, Manuelidis L. Use of biotinylated probe and in situ hybridization for light and electron microscopic localization of Po mRNA in myelin-forming Schwann cells. Histochemistry 1987;86:441–444.

41. Wessendorf MW. Characterization and use of multi-color fluorescence microscopic techniques. In: Björklund, A, Hökfelt T, Wouterlood FG, van den Pol AN, eds. Handbook of chemical neuroanatomy, vol 8. Analysis of neuronal microcircuits and synaptic interactions. Amsterdam: Elsevier, 1990;1–45.

42. Young WS III. Simultaneous use of digoxigenin- and radiolabeled oligodeoxyribonucleotide probes for hybridization histochemistry. Neuropeptides 1989;13:271–274.

43. Young WS III. In situ hybridization histochemistry. In: Björklund A, Hökfelt T, Wouterlood FG, van den Pol AN, eds. Handbook of chemical neuroanatomy, vol 8. Analysis of neuronal microcircuits and synaptic interactions. Amsterdam: Elsevier, 1990;481–512.

44. Young WS III, Kuhar MJ. A new method for receptor autoradiography: [3H] opioid receptors in rat brain. Brain Res 1979;179:255–270.

45. Zaborszky L, Heimer L. Combinations of tracer techniques, especially HRP and PHA-L, with transmitter identification for correlated light and electron microscopic studies. In: Heimer L, Zaborszky L, eds. Neuroanatomical tract-tracing methods 2. Recent progress. New York: Plenum, 1989

Back to Chapter